Part:BBa_K4650002:Design
spider silk protein MaSp1
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
MaSp1 amino acid sequence had several repeat amino acid sequences. After converting it into DNA sequence, our team designed the primer set to amplify MaSp1 via the PCR technique. However, the primer sets kept annealing to several places on the DNA sequence via the Snapgene app. After consulting with the company, they have suggested our team use different 3 nucleotide codons for the same amino acid to make DNA sequence not repeat much. Our team also added the start codon, ATG, at the 5'end of the MaSp1 DNA sequence so the RNA transcription would start from ATG on MaSp1 DNA
Source
Our team used Snapgene to convert the spider silk amino acid sequences, MaSp1, into DNA sequences, and then a genetic engineering company, Mission Biotech synthesized the DNA sequences. They synthesized MaSp1 on the pMA-RQ plasmid. Our team used the PCR technique to amplify MaSp1